Eficiência de Bacillus thuringiensis no controle de Plutella xylostella (Lepidoptera: Plutellidae) e compatibilidade com inseticidas

Rogério Teixeira Duarte; Sergio Antonio de Bortoli; Ricardo Antonio Polanczyk

doi:10.48017/Diversitas_Journal-v6i3-1849

Authors

Rogério Teixeira Duarte Universidade de Araraquara https://orcid.org/0000-0002-4908-6349
Sergio Antonio de Bortoli Universidade Estadual Paulista “Júlio de Mesquita Filho” https://orcid.org/0000-0003-0957-6164
Ricardo Antonio Polanczyk Universidade Estadual Paulista “Júlio de Mesquita Filho” https://orcid.org/0000-0003-0769-9902

DOI:

https://doi.org/10.48017/Diversitas_Journal-v6i3-1849

Abstract

ABSTRACT: The objective of this research was analyze the susceptibility of Plutella xylostella (L.) (Lepidoptera: Plutellidae) to isolates of Bacillus thuringiensis Berliner and to evaluate the compatibility of the most virulent isolates with insecticides registered to control cabbage pests. The efficiency of 13 isolates was evaluated through pathogenicity and virulence tests (LC₅₀ and LT₅₀) on second instar larvae of P. xylostella. The compatibility between the isolates and active ingredients was performed by the insecticide mixture in culture medium, and after this was inoculated an aliquot of microorganism suspension, being analyzed the number of spores / mL after seven days of entomopathogen growth. The isolates HD-1, HD-4, HD-11, HD-73, and T-07 were the most efficient on P. xylostella, and responsible to cause total mortality of immature, with LC₅₀ varying between 0.75 and 11.66 × 10³ spores / mL, and LT50 between 25.12 and 34.47 h. The isolates HD-1 and HD-4 showed neutral interaction with the active ingredient thiamethoxam, and positive interaction with the isolate T-07, with significant increase of sporulation. The other active ingredients were toxic in relation to the reproductive development of B. thuringiensis.

KEYWORDS: diamondback moth, entomopathogenic bacterium, biological control, chemical control.

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