Tissue Culture Adaptability of Sugarcane Meristematic Tissue to Different Culture Medium Preparation
DOI:
https://doi.org/10.48017/dj.v11iSpecial_2.3773Keywords:
sugarcane tissue culture, organic tissue culture, organic growth hormoneAbstract
Culture medium is one of the most important components to consider in the success of plant tissue culture. However, chemical-based plant growth hormones (PGH) and macronutrients are expensive. This challenge restricts the advantage of tissue culture as a technique to produce an elite clonal seedling. Thus, there is a need to find low-cost alternatives in replacing synthetic nutrients such as PGH and macronutrients. This study aims to know the adaptability of sugarcane meristematic tissue to the fermented plants waste solution developed by SUNN Plant Tissue Culture Laboratory that could replace the chemical-based PGH and macronutrients in terms of contamination rate, percentage of initiated cultures, and average days to shoot. The study was conducted in a Completely Randomized Design (CRD) with 4 treatments and replicated 4 times. The treatments were; MS Media, N6, organic-based macronutrients, and organic-based PGH. The data was analyzed using the Analysis of Variance using the STAR software. Data reveals that treatment with MS Media, and treatments with organic-based macronutrients and organic-based plant growth hormone showed comparable results in terms of contamination rate. However, data on the percentage of initiated cultures and average days to shoot were salient on chemical-based medium, yet initiations of cultures were recorded in organic-based media in the last few weeks. Thus, it is concluded and recommended to conduct further studies with higher concentration rates of fermented plant waste to validate results and to establish the right protocols for using fermented plant waste to be replaced as a tissue culture medium for better results.
Metrics
References
Ali, A., Naz, S. H. A. G. U. F. T. A., Siddiqui, F. A., & Iqbal, J. (2008). An efficient protocol for large scale production of sugarcane through micro propagation. Pakistan Journal of Botany, 40(1), 139.
Baksha, R., Alam, R., Karim, M.Z., Paul, S.K., Hossain, M.A., Miah, M.A.S., and Rahman,
A.B.M.M. (2002). In vitro Shoot Tip Culture of Sugar-cane (Saccharum officinarum) Variety Isd 28. Biotechnology, 1: 67-72.
Chimdessa, E. (2020). Composition and preparation of plant tissue culture medium. J. Tissue Cult. Bioeng, 3, 281-286.
Cursi, D. E., Hoffmann, H. P., Barbosa, G. V. S., Bressiani, J. A., Gazaffi, R., Chapola, R. G., ... & Carneiro, M. S. (2022). History and current status of sugarcane breeding, germplasm development and molecular genetics in Brazil. Sugar Tech, 24(1), 112-133.
Da Silva, A. L. L., Costa, J. D. L., Gollo, A. L., Dos Santos, J. D., Forneck, H. R., Biasi, L. A., ... & Soccol, C. R. (2014). Development of a vinasse culture medium for plant tissue culture. Pak J Bot, 46(6), 2195-2202.
Dash, M., Chiellini, F., Ottenbrite, R. M., & Chiellini, E. (2011). Chitosan—A versatile semi- synthetic polymer in biomedical applications. Progress in polymer science, 36(8), 981-1014.
Daud, N., Taha, R. M., Noor, N. N. M., & Alimon, H. (2011). Provision of low-cost media options for in vitro culture of Celosia sp. African Journal of Biotechnology, 10(80), 18349- 18355.
Debergh, PC and Read, PE. (1991). Micropropagation. In Micropropagation. (pp.1-13). Springer, Dordrecht.
Duda-Chodak A, and Tarko T. (2007). Antioxidant Properties of Different Fruit Seeds and Peels. Acta Scientiarum Polonorum Technologia Alimentaria. (pp. 29-36).
Gomez, K. A., & Gomez, A. A. (1984). Statistical procedures for agricultural research. John Wiley & Sons.
Habiba, U., Reza, S., Saha, M. L., Khan, M. R., & Hadiuzzaman, S. (2002). Endogenous bacterial contamination during in vitro culture of table banana: Identification and prevention. Plant Tissue Cult, 12(2), 117-124.
Hamza, T. A., & Alebjo, A. L.(2017). Sugarcane (Saccharum Officinarum L) Tissue Culture In Ethiopia: Opportunities For Ethiopia’s Sugar Industries. International Journal Of Scientific & Technology Research Volume 6, Issue 08. www.ijstr.org,
Ibrahim, M., Tolera, B., Biruma, M., Bedada, A., Shimelis, D., Aman, J., ... & Negi, T. (2016). Evaluation of tissue culture raised sugarcane planting materials against their donor conventional seed sources as initial source of seed cane at Tendaho Sugar Development Project, North-Eastern Ethiopia. Journal of Horticulture, 1-4.
Idowu, P. E., Ibitoye, D. O., & Ademoyegun, O. T. (2009). Tissue culture as a plant production technique for horticultural crops. African Journal of Biotechnology, 8(16).
Jaafar, F., Awal, A., Abdullah, S., Ismail, F., Sidik, N. J., & Yusuf, N. A. (2015). Bio-potential of fermented fruit waste solutions on in vitro seed germination and regeneration of Lycium barbarum and Aquilaria malaccensis Lamk. Malaysian Applied Biology, 44(3), 127-133.
Khan, I. A., Dahot, M. U., Seema, N., Yasmeen, S., Bibi, S., Raza, G., and Naqvi, M. H. (2009). Direct regeneration of sugarcane plantlets: a tool to unravel genetic heterogeneity. Pak. J. Bot, 41(2), 797-814.
Khirul Anuar NA, Mohd Shaain UH, Awal A, and Yusuf NA. (2016). Selected Fruits Peel as an Alternative Low Cost Medium Component for In Vitro Micro propagation of Musa acuminata cv. Berangan (AAA). Paper presented at regional Conference on Science, Technology and Social Sciences. Copthorne Hotel, Cameron Highlands, Brinchang, Malaysia.
Kodym A and Zapata-Arias FJ. (2001). Low Cost Alternatives for the Micropropagation of Banana. In Plant Cell, Tissue and Organ Culture. 2001 Jul 1:66(1) (pp. 67-71)
Kurimella, Ravindra. (2016). Re: Why MS media is most frequently used for plant tissue culture?. Retrieved from: https://www.researchgate.net/post/Why-MS-media-is-most-frequently- used-for-plant-tissue-culture/579a103eeeae3915e06e3f38/citation/download.
Leifert, C.,& Cassells, A. C. (2001).Microbial Hazards in Plant Tissue and Cell Cultures. In Vitro Cellular ＆ Developmental Biology. Plant, Vo1. 37, No. 2,pp.133-138.
Mangla, Y., Khanduri, P., & Gupta, C. K. (2022). Reproductive Biology of Angiosperms: Concepts and Laboratory Methods. Cambridge University Press.
Murashige, T. (1974). Plant propagation through tissue cultures. Annual review of plant physiology, 25(1), 135-166.
Nadirah, M. N. A., Awal, A., Azma, Y. N., & Sulaiman, A. (2019, September). POME: An alternative nutrient source for bio-organic plant tissue culture media. In IOP Conference Series: Earth and Environmental Science (Vol. 327, No. 1, p. 012029). IOP Publishing.
Salokhe, S. (2021). Development of an efficient protocol for production of healthy sugarcane seed cane through Meristem culture. Journal of Agriculture and Food Research, 4, 100126.
Savangikar VA. (2004). Role of Low Cost in Tissue Culture. In Low Cost Options for Tissue Culture Technology in Developing Countries. Proceedings of a Technical Meeting Organized by the Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture.(pp. 11-15).
Singh, J., & Singh, R. (2015). Sugarcane seed production: Indian scenario. J. Biotechnol. Crop Sci, 4, 43-55.
Singh, R. (2003). Tissue culture studies of sugarcane. Thapar Institute of Engineering and Technology, Patiala, India.
Solomon, S., Swapna, M., Xuan, V. T., & Mon, Y. Y. (2016). Development of sugar industry in ASEAN countries. Sugar Tech, 18(6), 559-575.
Wojtania A, Pulawska J, Gabryszewska E (2005) Identification and elimination of bacterial contaminants from Pelargonium tissue cultures. J Fruit Ornam Plant Res 13:101–108
Yaseen, M., Ahmad, T., Sablok, G., Standardi, A., & Hafiz, I. A. (2013). Role of carbon sources for in vitro plant growth and development. Molecular biology reports, 40, 2837-2849.
Yutaka, T., Y. Tomohiro, M. Toshikazu and O. Takeshi. (1998). Plant regeneration via shoot organogenesis from cotyledons in two wild Cucumis species, C. figarei and C. metuliferus. Jap. Agric. Res. Q., 32: 281-286.
Yusuf NA. (2012) Bio-organic Tissue Culture Media for Pitaya’s (Hylocereus polyrhizus) Shoot Growth (Master-degree thesis). Universiti Teknologi MARA (UiTM), Shah Alam, Selangor, Malaysia.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 Ian Reymart Alfanza, Nyle Pardillo
This work is licensed under a Creative Commons Attribution 4.0 International License.
The Diversitas Journal expresses that the articles are the sole responsibility of the Authors, who are familiar with Brazilian and international legislation.
Articles are peer-reviewed and care should be taken to warn of the possible incidence of plagiarism. However, plagiarism is an indisputable action by the authors.
The violation of copyright is a crime, provided for in article 184 of the Brazilian Penal Code: “Art. 184 Violating copyright and related rights: Penalty - detention, from 3 (three) months to 1 (one) year, or fine. § 1 If the violation consists of total or partial reproduction, for the purpose of direct or indirect profit, by any means or process, of intellectual work, interpretation, performance or phonogram, without the express authorization of the author, the performer, the producer , as the case may be, or whoever represents them: Penalty - imprisonment, from 2 (two) to 4 (four) years, and a fine. ”
