Avaliação da sensibilidade de Colletotrichum spp. a Trichoderma harzianum

Jackeline Laurentino da Silva; Jaqueline Figueredo de Oliveira Costa; Cecilia Hernandez Ramirez; Maria Jussara dos Santos da Silva; Maria Hilma dos Santos; Gaus Silvestre de Andrade Lima; Iraildes Pereira Assunção

doi:10.48017/dj.v10i1.3065

Authors

Jackeline Laurentino da Silva Federal University of Alagoas, Brazil https://orcid.org/0000-0002-9870-3673
Jaqueline Figueredo de Oliveira Costa Federal University of Alagoas, Brazil https://orcid.org/0000-0003-0121-699X
Cecilia Hernandez Ramirez State University of Alagoas, Brazil https://orcid.org/0000-0002-8639-0470
Maria Jussara dos Santos da Silva State University of Alagoas, Brazil https://orcid.org/0000-0001-9418-854X
Maria Hilma dos Santos State University of Alagoas, Brazil https://orcid.org/0000-0001-8592-6977
Gaus Silvestre de Andrade Lima State University of Alagoas, Brazil
Iraildes Pereira Assunção State University of Alagoas, Brazil

DOI:

https://doi.org/10.48017/dj.v10i1.3065

Keywords:

antracnose, biofungicides, biological fungicides, mycoparasitism, mycoparasitism Passiflora

Abstract

The objective of this study was to evaluate in vitro the effects of T. harzianum on five species of Colletotrichum. For the pairing test, Colletotrichum spp. was inoculated in synthetic PDA medium and after 72 hours the antagonist T. harzianum was added, in the opposite direction to the phytopathogens. To evaluate the action of metabolites, Colletotrichum species were inoculated in the center of Petri dishes, separately and after 48 hours T. harzianum was inoculated in plates containing synthetic PDA medium. The bases of the plates containing antagonist and phytopathogens were joined and sealed. The mycelial growth index (MGI) and the percentage of mycelial growth inhibition (PMGI) were estimated. When evaluating the antagonism of T. harzianum to Colletotrichum spp. in the pairing test and volatile metabolites, it was observed that all Colletotrichum species presented PMGI below 31% in both tests. In the MGI for the matching test, it was observed that C. tropicale was the most sensitive species (1.87 cm) at the concentrations of 1 and 1.5 L/ha, while in the volatile metabolites test it was C. plurivorum (3.21 cm). T. harzianum is a promising species for the reduction of Colletotrichum spp. associated with anthracnose in passion fruit crops.

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Author Biographies

Jackeline Laurentino da Silva, Federal University of Alagoas, Brazil

0000-0003-4222-0805; Federal University of Alagoas (UFAL)/PhD in the Postgraduate Course in Plant Protection, Campus of Engineering and Agricultural Sciences. Rio Largo, Alagoas (AL), BRAZIL. jackeline.laurentino@outlook.com

Jaqueline Figueredo de Oliveira Costa, Federal University of Alagoas, Brazil

0000-0003-0121-699X; Universidad Federal de Alagoas (UFAL), Investigador del Campus de Ingeniería y Ciencias Agrarias. Rio Largo, Alagoas (AL), BRASIL. jaquelinefigueredo@hotmail.com

Cecilia Hernandez Ramirez, State University of Alagoas, Brazil

0000-0002-8639-0470; Federal University of Alagoas (UFAL)/PhD student in the Postgraduate course in Plant Protection at the Campus of Engineering and Agricultural Sciences. Rio Largo, Alagoas (AL), BRAZIL. cecih967@gmail.com

Maria Jussara dos Santos da Silva, State University of Alagoas, Brazil

0000-0001-9418-854X; Federal University of Alagoas (UFAL)/PhD in the Postgraduate Course in Plant Protection of the Campus of Engineering and Agricultural Sciences. Rio Largo, Alagoas (AL), BRAZIL. maria_jussara@hotmail.com.br;

Maria Hilma dos Santos, State University of Alagoas, Brazil

0000-0001-8592-6977; Federal University of Alagoas (UFAL)/Master's student in Plant Protection at the Campus of Agricultural Sciences and Engineering. Rio Largo, Alagoas (AL), BRAZIL. hilma2050@gmail.com

Gaus Silvestre de Andrade Lima, State University of Alagoas, Brazil

0000-0003-2910-5896; Federal University of Alagoas (UFAL), Prof. Dr. of the Campus of Engineering and Agricultural Sciences. Rio Largo, Alagoas (AL), BRAZIL. gaus@ceca.ufal.br.

Iraildes Pereira Assunção, State University of Alagoas, Brazil

0000-0001-5087-0168; Federal University of Alagoas (UFAL), Professor of the Campus of Engineering and Agricultural Sciences. Rio Largo, Alagoas (AL), BRAZIL. iraildes.assuncao@ceca.ufal.br

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