Actinobacteria with agricultural biotechnological potential isolated from fermented organic fertilizer (Bokashi)
DOI:
https://doi.org/10.48017/dj.v6i4.1946Abstract
Bokashi is a type of organic fermented product formed by fungi and bacteria isolated from forests and which coexist synergistically in a liquid fermentative medium enriched with a sugar source. Among these microorganisms, some species of actinobacteria may have biotechnological potential to be used in agriculture. In this sense, the present work aimed to isolate and identify actinobacteria with agricultural biotechnological potential present in fermented organic fertilizer (Bokashi). Bokashi was produced by capturing microorganisms present in the forest soil. Actinobacteria were isolated through successive dilutions of Bokashi samples and grown in culture media Czapeck Modified (MC), Arginine Vitamin Agar (AY*), Inorganic Salt – starch Agar (ISP-4) containing antifungal, and incubated at 30° C for 21 days. Micromorphological analysis and identification was performed using the microculture on slides technique. In all, 22 strains of actinobacteria were isolated and identified, of which 77.27% were from the AY* medium; 13.64% of ISP-4 medium and 9.1% of MC medium. The presumptive identification of the genders showed that the strains were distributed between: 45.45% Nocardia; 36.36% Streptomyces; 9.1% Terrabacter; 4.54% Micromonospora; and 4.54% Mycobacterium.
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