an Identification of endophytic fungi associated with plants from the Alagoas Semiarid
DOI:
https://doi.org/10.48017/dj.v9i2.2859Keywords:
micro cultivation, riparian forests, filamentous fungiAbstract
In the semi-arid region of Alagoas, there are difficulties for small producers to provide food for animal production, water scarcity is one of the challenges faced and causes significant damage. However, lack of conservation and lack of knowledge are factors that attribute to overheating and lack of rainfall. By combining these factors, there is a need to study and prove the importance of these tree species not only in maintaining natural ecosystems, but also in improving the quality of animal production. Through the micro cultivation method, the primary identification of the endophytes was carried out, according to the Anvisa Manual (2004) for the identification of filamentous fungi. Morphological and microscopic aspects and sporulation patterns were observed. In sterile Petri dishes were slided with APD agar blocks. After sowing the endophytes in the culture media, they were covered with coverslips, after growth in 3 and 5 days, the coverslips were stained with methylene blue and, based on observation under a microscope, the sporulation structures were consulted by means of the bibliographical references such as atlases and manuals for identifying the genus of endophytes.
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References
Brambilla, M. (2016). Análise econômica de cenários de operação de reservatórios considerando o hidrograma ambiental para o baixo curso do rio São Francisco. Dissertação apresentada à Escola Politécnica da Universidade Federal da Bahia, em Meio Ambiente, Águas e Saneamento, 157.
Costa, F. G. P., Clementino, R. H., Jácome, I. M. T. D., do Nascimento, G. A. J., & Pereira, W. E. (2004). Utilização de um complexo multienzimático em dietas de frangos de corte. Ciência Animal Brasileira, 5(2), 63-71.
Dalla Rosa, M. (2013). A Importância das Matas Ciliares na Tutela Jurídica dos Corpos D’água. Journal of Law and Sustainable Development, 1(1), 53-84.
El-Sharkawy, I., Sherif, S., Abdulla, M., & Jayasankar, S. (2017). Plum fruit development occurs via gibberellin–sensitive and–insensitive DELLA repressors. PLoS One, 12(1), e0169440.
Freire, K. T. L. S., Araújo, G. R., Bezerra, J. D. P., Barbosa, R. N., Silva, D. C. V., Svedese, V. M., ... & Souza-Motta, C. M. (2015). Fungos endofíticos de Opuntia ficus-indica (L.) Mill.(Cactaceae) sadia e infestada por Dactylopius opuntiae (Cockerell, 1896)(Hemiptera: Dactylopiidae). Revista Gaia Scientia, 9, 104-110.
Gunatilaka, A. L. (2006). Natural products from plant-associated microorganisms: distribution, structural diversity, bioactivity, and implications of their occurrence. Journal of natural products, 69(3), 509-526.
Hermosa, R., Cardoza, R. E., Rubio, M. B., Gutiérrez, S., & Monte, E. (2014). Secondary metabolism and antimicrobial metabolites of Trichoderma. In Biotechnology and biology of Trichoderma (pp. 125-137). Elsevier.
Jakievicius, M. (2011). Secretaria do Meio Ambiente / Coordenadoria de Educação Ambiental. Matas Ciliares e o Meio Ambiente Rural: uma proposta de trabalho para educadores. São Paulo: SMA/CEA, 152.
Krishnamoorthy, U., & Krishnappa, P. (1996). Effect of feeding yeast culture (Yea-sacc1026) on rumen fermentation in vitro and production performance in crossbred dairy cows. Animal Feed Science and Technology, 57(3), 247-256.
Kern, M. E., & Blevins, K. S. (1999). Micologia Médica. 2 Edição. Editora Premier, São Paulo, 256.
Marengo, J. A., Alves L, M., Beserra, E. A., & Lacerda, F. F. (2011). Variabilidade e mudanças climáticas no semiárido brasileiro. Recursos Hídricos em regiões Áridas e Semiáridas. Campina Grande - PB. 385–422.
Melo, I. S. (1998). Agentes microbianos de controle de fungos fitopatogenicos. Em I. S. Melo & J. L. Azevedo (Eds.), Controle biológico.
Oliveira, I. N. D. (2019). Detecção de pigmentos e metabólitos secundários bioativos de extratos de fungos endofíticos associados à Manilkara salzmannii, planta da restinga de Salvador, Ba.
Owen, D., Williams, A. P., Griffith, G. W., & Withers, P. J. (2015). Use of commercial bio-inoculants to increase agricultural production through improved phosphrous acquisition. Applied Soil Ecology, 86, 41-54.
Paulino, R. D. C., Henriques, G. P., Moura, O. N., Coelho, M. D. F. B., & Azevedo, R. A. (2012). Medicinal plants at the Sítio do Gois, Apodi, Rio Grande do Norte State, Brazil. Revista Brasileira de Farmacognosia, 22, 29-39.
Petrini, O. (1991). Fungal endophytes of tree leaves. In Microbial ecology of leaves (pp. 179-197). New York, NY: Springer New York.
Pollard, D. J., & Woodley, J. M. (2007). Biocatalysis for pharmaceutical intermediates: the future is now. TRENDS in Biotechnology, 25(2), 66-73.
Samson, R. A., & Varga, J. (2009). Molecular systematics of Aspergillus and its teleomorphs. Em Aspergillus: molecular biology and genomics (pp. 19–40). Horizon Press.
Santos, J. É. M. (2021). Uma análise territorial sobre o uso e a preservação das plantas medicinais nas comunidades Povoado Caraíbas do Lino, Delmiro Gouveia/AL, e Serra das Viúvas, Água Branca/AL.
Strobel, Gary; Daisy, Bryn. Bioprospecting for microbial endophytes and their natural products. Microbiology and molecular biology reviews, v. 67, n. 4, p. 491-502, 2003.
Thambugala, K. M., Daranagama, D. A., Phillips, A. J., Kannangara, S. D., & Promputtha, I. (2020). Fungi vs. fungi in biocontrol: An overview of fungal antagonists applied against fungal plant pathogens. Frontiers in cellular and infection microbiology, 10, 604923.
Ulhoa, C. J., & Peberdy, J. F. (1992). Purification and some properties of the extracellular chitinase produced by Trichoderma harzianum. Enzyme and Microbial Technology, 14(3), 236-240.
Wallace, R. J. (1994). Ruminal microbiology, biotechnology, and ruminant nutrition: progress and problems. Journal of Animal Science, 72(11), 2992-3003.
Zaneti, V. (2012). Produção de xilanases por Aspergillus niger utilizando planejamento experimental: purificação de xilanase, 74.
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