Carboxymethylcellulose and starch as vehicles for microbial inoculants.
DOI:
https://doi.org/10.48017/dj.v11iSpecial_2.3781Keywords:
Phosphate-solubilizing bacteria, polymeric mixture, BacillusAbstract
Inoculants are formulations that require a high-quality carrier to ensure the concentration of active cells, as well as to tolerate variations in temperature, humidity, aeration, and storage time. Therefore, this study aimed to evaluate the efficiency of a carboxymethylcellulose-starch polymer mixture as a vehicle for inoculating Bacillus subtilis and Bacillus pumilus strains. The preparations were stored at room temperature, and the survival of the microorganisms was evaluated at 15, 30, 45, and 60 days after inoculation. The test was performed in triplicate on nutrient agar medium, with counting after 24 hours of incubation. Significant differences in cell viability were detected in relation to storage time. For both strains, viability was maintained for up to 60 days. Differences were observed between the two strains regarding the increase in the number of CFUs; for B. pumilus, the cell concentration was maintained for up to 30 days of storage, while for B. subtilis, a significant difference was observed after 15 days compared to the initial time. The polymer mixture maintained cell viability of 109 CFU/mL for 60 days at room temperature, demonstrating its potential as a sustainable tool to increase agricultural productivity with less environmental impact.
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