Evolução da Pesquisa sobre Fungos Endofíticos Associados a Plantas: Uma Análise Cienciométrica e Bibliométrica (2015–2025)

Maria do Socorro Mascarenhas; Gilberto José Arruda; Margareth Batistote

doi:10.48017/dj.v11i3.3956

Authors

Maria do Socorro Mascarenhas Mato Grosso do Sul State University/UEMS, Dourados, Mato Grosso do Sul (MS), Brazil https://orcid.org/0000-0002-5343-4502
Gilberto José Arruda Mato Grosso do Sul State University/UEMS, Dourados, Mato Grosso do Sul (MS), Brazil https://orcid.org/0000-0001-8297-3620
Margareth Batistote Mato Grosso do Sul State University/UEMS, Dourados, Mato Grosso do Sul (MS), Brazil

DOI:

https://doi.org/10.48017/dj.v11i3.3956

Keywords:

Symbiotic interaction, Host plants, Multivariate analysis

Abstract

Endophytic fungi associated with plants have received increasing scientific attention due to their ecological relevance and high biotechnological potential, especially for the production of bioactive metabolites with agricultural, pharmacological, and environmental applications. This study aimed to analyze patterns, trends, and gaps in scientific production on endophytic fungi associated with plants from 2015 to 2025, using a descriptive scientometric approach. Articles indexed in the Web of Science Core Collection, selected based on predefined criteria, were analyzed. Data were evaluated using descriptive statistics and multivariate analyses, including Principal Coordinate Analysis (PCoA). The results showed continuous growth in scientific production, concentrated in countries such as China, Brazil, India, and the United States. An asymmetrical pattern was observed in the selection of host botanical families, with a predominance of Poaceae, Fabaceae, Bignoniaceae, Meliaceae, Zingiberaceae, and Asteraceae, indicating taxonomic biases associated with the economic and medicinal relevance of these plants. PCoA demonstrated that the selection of botanical families follows scientific trends and temporal dynamics throughout the analyzed period. It is concluded that, although the area is consolidated, expanding the taxonomic and geographic scope of research is fundamental to reducing knowledge gaps and enhancing the discovery of new endophytic fungi and bioactive metabolites.

Author Biographies

Maria do Socorro Mascarenhas, Mato Grosso do Sul State University/UEMS, Dourados, Mato Grosso do Sul (MS), Brazil

0000-0002-5343-4502; Mato Grosso do Sul State University/UEMS, Dourados, Mato Grosso do Sul (MS), Brazil. maria_mascarenhas@outlook.com

Gilberto José Arruda, Mato Grosso do Sul State University/UEMS, Dourados, Mato Grosso do Sul (MS), Brazil

0000-0001-8297-3620; Mato Grosso do Sul State University/UEMS, Dourados, Mato Grosso do Sul (MS), Brazil. arruda@uems.br

Margareth Batistote, Mato Grosso do Sul State University/UEMS, Dourados, Mato Grosso do Sul (MS), Brazil

0000-0001-9865-2362; Mato Grosso do Sul State University/UEMS, Dourados, Mato Grosso do Sul (MS), Brazil. margarethbatistote@gmail.com

References

Adeleke, B. S., & Babalola, O. O. (2021). Pharmacological potential of fungal endophytes associated with medicinal plants: A review. Journal of Fungi, 7(2), 147. https://doi.org/10.3390/jof7020147

Ancheeva, E., Daletos, G., & Proksch, P. (2020). Bioactive secondary metabolites from endophytic fungi. Current Medicinal Chemistry, 27(11), 1836–1854. https://doi.org/10.2174/0929867326666190916144709

Ansabayeva, A., Makhambetov, M., Rebouh, N. Y., Abdelkader, M., Saudy, H. S., Hassan, K. M., Nasser, M. A., Ali, M. A. A., & Ebrahim, M. (2025). Plant growth-promoting microbes for resilient farming systems: Mitigating environmental stressors and boosting crops productivity—A review. Horticulturae, 11(3), 260. https://doi.org/10.3390/horticulturae11030260

Baron, N. C., & Rigobelo, E. C. (2022). Endophytic fungi: A tool for plant growth promotion and sustainable agriculture. Mycology, 13(1), 39–55. https://doi.org/10.1080/21501203.2021.1945699

Bezerra, V. H. S., Cardoso, S. L., Fonseca-Bazzo, Y., Silveira, D., Magalhães, P. O., & Souza, P. M. (2021). Protease produced by endophytic fungi: A systematic review. Molecules, 26(22), 7062. https://doi.org/10.3390/molecules26227062

Bogas, A. C., Cruz, F. P. N., Lacava, P. T., & Sousa, C. P. (2024). Endophytic fungi: An overview on biotechnological and agronomic potential. Brazilian Journal of Biology, 84, e258557. https://doi.org/10.1590/1519-6984.258557

Coertjens, N. C., Mascarenhas, M. S., & Batistote, M. (2024). Brazilian endophytic fungi: Biotechnological potential in evidence. Diversitas Journal, 9(3), 1253–1266. https://doi.org/10.48017/dj.v9i3.2840

Devi, R., Verma, R., Dhalaria, R., Kumar, A., Kumar, D., Puri, S., Thakur, M., Chauhan, S., Chauhan, P. P., Nepovimova, E., & Kuca, K. (2023). A systematic review on endophytic fungi and its role in the commercial applications. Planta, 257(4), 70. https://doi.org/10.1007/s00425-023-04087-2

Dos Reis, J. B. A., Lorenzi, A. S., Pinho, D. B., Cortelo, P. C., & do Vale, H. M. M. (2024). The hidden treasures in endophytic fungi: A comprehensive review on the diversity of fungal bioactive metabolites, usual analytical methodologies, and applications. Archives of Microbiology, 206(4), 185. https://doi.org/10.1007/s00203-024-03911-x

Dos Reis, J. B. A., Pappas Junior, G. J., Lorenzi, A. S., Pinho, D. B., Costa, A. M., Bustamante, M. M. D. C., & do Vale, H. M. M. (2023). How deep can the endophytic mycobiome go? A case study on six woody species from the Brazilian Cerrado. Journal of Fungi, 9(5), 508. https://doi.org/10.3390/jof9050508

Dos Santos, I. R., Mohamed, T. A., Borges, L. L., Abo Nahas, H. H., Abdel-Azeem, M. A., Carvalho, D. D. C., Bezerra, J. P., Gupta, V. K., Xavier-Santos, S., Mossa, M. I., & Abdel-Azeem, A. M. (2022). The global tendency in the research of biological activity in endophytic fungi: A scientometric analysis. Current Research in Environmental & Applied Mycology, 12(1), 1–14. https://doi.org/10.5943/cream/12/1/1

Gakuubi, M. M., Munusamy, M., Liang, Z.-X., & Ng, S. B. (2021). Fungal endophytes: A promising frontier for discovery of novel bioactive compounds. Journal of Fungi, 7(10), 786. https://doi.org/10.3390/jof7100786

Gao, Y., Xu, Y., Dong, Z., Guo, Y., Luo, J., Wang, F., Yan, L., & Zou, X. (2025). Endophytic fungal diversity and its interaction mechanism with medicinal plants. Molecules, 30(5), 1028. https://doi.org/10.3390/molecules30051028

Goyal, J., Upadhayay, P., Talukdar, A. D., Yadav, P., Banerjee, P., Shakya, A., ... & Rai, N. (2025). Proteomics of Fungal Endophytes: Trends and Insight for Biotechnology. In Multi-omics Approach to Investigate Endophyte Diversity (pp. 33-72). Singapore: Springer Nature Singapore.

Guo, S., Ye, G., Liu, W., Liu, R., Liu, Z., & Ma, Y. (2024). Environmental factors drive the biogeographic pattern of Hippophae rhamnoides root endophytic fungal diversity in the arid regions of Northwest China. Journal of Fungi, 10(10), 679. https://doi.org/10.3390/jof10100679

Harman, G. E., Doni, F., Khadka, R. B., & Uphoff, N. (2021). Endophytic strains of Trichoderma increase plants’ photosynthetic capability. Journal of Applied Microbiology, 130(2), 529–546. https://doi.org/10.1111/jam.14368

Hashem, A. H., Attia, M. S., Kandil, E. K., Fawzi, M. M., Abdelrahman, A. S., Khader, M. S., Khodaira, M. A., Emam, A. E., Goma, M. A., & Abdelaziz, A. M. (2023). Bioactive compounds and biomedical applications of endophytic fungi: A recent review. Microbial Cell Factories, 22(1), 107. https://doi.org/10.1186/s12934-023-02118-x

Hu, A. G. Z. (2020). Public funding and the ascent of Chinese science: Evidence from the National Natural Science Foundation of China. Research Policy, 49(5), 103983. https://doi.org/10.1016/j.respol.2020.103983

Jha, P., Kaur, T., Chhabra, I., Panja, A., Paul, S., Kumar, V., & Malik, T. (2023). Endophytic fungi: Hidden treasure chest of antimicrobial metabolites interrelationship of endophytes and metabolites. Frontiers in Microbiology, 14, 1227830. https://doi.org/10.3389/fmicb.2023.1227830

Kumar, G., Chandra, P., & Choudhary, M. (2017). Endophytic fungi: A potential source of bioactive compounds. Chem. Sci. Rev. Lett, 6, 2373-2381.

Lee, S., Nguyen, Q. N., Phung, H. M., Shim, S. H., Kim, D., Hwang, G. S., & Kang, K. S. (2021). Preventive effects of anthraquinones isolated from an endophytic fungus, Colletotrichum sp. JS-0367 in tumor necrosis factor-α-stimulated damage of human dermal fibroblasts. Antioxidants, 10(2), 200. https://doi.org/10.3390/antiox10020200

Li, Q. X., Lin, F. C., & Su, Z. Z. (2025). Endophytic fungi—Big player in plant-microbe symbiosis. Current Plant Biology, 42, 100481. https://doi.org/10.1016/j.cpb.2025.100481

Majhi, P., Pradhan, U., Toppo, A., & Shukla, A. K. (2025). Fungal endophytes: An insight into diversity, stress tolerance, biocontrol and plant growth-promoting potentials. Current Microbiology, 82(6), 283. https://doi.org/10.1007/s00284-025-04266-2

Malicka, M., Magurno, F., & Piotrowska-Seget, Z. (2022). Plant association with dark septate endophytes: When the going gets tough (and stressful), the tough fungi get going. Chemosphere, 302, 134830. https://doi.org/10.1016/j.chemosphere.2022.134830

Manganyi, M. C., & Ateba, C. N. (2020). Untapped potentials of endophytic fungi: A review of novel bioactive compounds with biological applications. Microorganisms, 8(12), 1934. https://doi.org/10.3390/microorganisms8121934

Mattoo, A. J., & Nonzom, S. (2021). Endophytic fungi: Understanding complex cross-talks. Symbiosis, 83(3), 237–264. https://doi.org/10.1007/s13199-020-00744-2

Mayrhofer, B. F., Iantas, J., Noriler, S. A., Ponomareva, L. V., Thorson, J. S., Rohr, J., Shaaban, K. A., & Glienke, C. (2024). Highly diverse endophytic fungi from Serra do Amolar-Pantanal (Brazil) producing bioactive secondary metabolites against phytopathogens. Frontiers in Microbiology, 15, 1501182. https://doi.org/10.3389/fmicb.2024.1501182

Mendieta-Brito, S., Sayed, M., Hamza, F. A., Son, E., Kim, D. S., Plata, G., ... & Pyo, S. H. (2025). Identification, characterization, antimicrobial activity and biocontrol potential of four endophytic fungi isolated from Amazonian plants. Scientific Reports, 15(1), 39361.

Mulyani, Y., Sinaga, S. E., & Supratman, U. (2023). Phytochemistry and biological activities of endophytic fungi from the Meliaceae family. Molecules, 28(2), 778. https://doi.org/10.3390/molecules28020778

Noriler, S. A., Savi, D. C., Aluizio, R., Palácio-Cortes, A. M., Possiede, Y. M., & Glienke, C. (2018). Bioprospecting and structure of fungal endophyte communities found in the Brazilian biomes, Pantanal, and Cerrado. Frontiers in Microbiology, 9, 1526. https://doi.org/10.3389/fmicb.2018.01526

Papik, J., Folkmanova, M., Polivkova-Majorova, M., Suman, J., & Uhlik, O. (2020). The invisible life inside plants: Deciphering the riddles of endophytic bacterial diversity. Biotechnology Advances, 44, 107614. https://doi.org/10.1016/j.biotechadv.2020.107614

Prajapati, C., Rai, S. N., Singh, A. K., Chopade, B. A., Singh, Y., Singh, S. K., Haque, S., Prieto, M. A., & Ashraf, G. M. (2025). An update of fungal endophyte diversity and strategies for augmenting therapeutic potential of their potent metabolites: Recent advancement. Applied Biochemistry and Biotechnology, 197(5), 2799–2866. https://doi.org/10.1007/s12010-024-05098-9

Rai, N., Kumari Keshri, P., Verma, A., Kamble, S. C., Mishra, P., Barik, S., Kumar Singh, S., & Gautam, V. (2021). Plant associated fungal endophytes as a source of natural bioactive compounds. Mycology, 12(3), 139–159. https://doi.org/10.1080/21501203.2020.1870579

Ramatsitsi, N., & Manyevere, A. (2025). Roles of endophytic fungi in plant resilience under abiotic stress: A mechanistic review with implications for climate-smart agriculture. Plant Signaling & Behavior, 20(1), 2578712. https://doi.org/10.1080/15592324.2025.2578712

Santoyo, G. (2025). Advances in the plant microbiome: Rhizosphere, endosphere, and phyllosphere. Microorganisms, 13(11), 2581. https://doi.org/10.3390/microorganisms13112581

Satheesan, J., & Sabu, K. K. (2020). Endophytic fungi for a sustainable production of major plant bioactive compounds. In Plant-derived bioactives: Production, properties and therapeutic applications (pp. 195-207). Singapore: Springer Singapore.

Sena, L., Mica, E., Valè, G., Vaccino, P., & Pecchioni, N. (2024). Exploring the potential of endophyte-plant interactions for improving crop sustainable yields in a changing climate. Frontiers in Plant Science, 15, 1349401. https://doi.org/10.3389/fpls.2024.1349401

Sharma, S. B., Raverkar, K. P., Wani, S. P., Bagyaraj, D. J., Kannepalli, A., Kandula, D. R., ... & Sharma, R. (2025). Role of the Plant–Microbiome Partnership in Environmentally Harmonious 21st Century Agriculture. Microorganisms, 13(12), 2839.

Sharma, S., Dhar, M. K., & Kaul, S. (2023). Antagonistic, plant growth promoting and extracellular hydrolytic enzyme activity of fungal endophytes of Dioscorea bulbifera L. Biocatalysis and Agricultural Biotechnology, 50, 102694. https://doi.org/10.1016/j.bcab.2023.102694

Silva, R. M. F., Neto, W. P. P., Oliveira, R. J. V., Bezerra, J. D. P., Bezerra, J. L., de Lima, V. X., Vieira, L. C., Tabosa, J. N., Souza-Motta, C. M., & Silva, G. A. (2023). Effect of climate and phenological stage on fungal endophytes community in Sorghum bicolor leaves. Mycological Progress, 22(3), 19. https://doi.org/10.1007/s11557-023-01870-z

Singh, M., Naskar, A., Rupashree, A., Rajput, M., & Singh, V. K. (2023). Analysis of endophytic microbes harboring in medicinal plants of Himalayan region with their medicinal properties. Biocatalysis and Agricultural Biotechnology, 53, 102857. https://doi.org/10.1016/j.bcab.2023.102857

Stakelienė, V., Pašakinskienė, I., Matijošiūtė, S., Martūnas, J., & Štukėnienė, G. (2025). Identifying root-associated endophytic fungi and bacteria in Festuca and Lolium grasses from a site in Lithuania. Microorganisms, 13(4), 799. https://doi.org/10.3390/microorganisms13040799

Thangavel, M., Sneha, M. J. X., Mani, I., Surendrababu, A., Rajapriya, P., Arulselvan, P., Alarfaj, A. A., Thangavelu, I., & Pandi, M. (2025). Diversity of endophytic fungi in plant species: Traditional vs. high-throughput sequencing approaches. Chemistry & Biodiversity, e202402792. https://doi.org/10.1002/cbdv.202402792

Vandenkoornhuyse, P., Quaiser, A., Duhamel, M., Le Van, A., & Dufresne, A. (2015). The importance of the microbiome of the plant holobiont. New Phytologist, 206(4), 1196–1206. https://doi.org/10.1111/nph.13312

Verma, A., Shameem, N., Jatav, H. S., Sathyanarayana, E., Parray, J. A., Poczai, P., & Sayyed, R. Z. (2022). Fungal endophytes to combat biotic and abiotic stresses for climate-smart and sustainable agriculture. Frontiers in Plant Science, 13, 953836. https://doi.org/10.3389/fpls.2022.953836

Verma, V., Srivastava, A., Garg, S. K., Singh, V. P., & Arora, P. K. (2024). Incorporating omics-based tools into endophytic fungal research. Biotechnology Notes, 5, 1–7. https://doi.org/10.1016/j.biotno.2023.12.006

Wen, J., Okyere, S. K., Wang, S., Wang, J., Xie, L., Ran, Y., & Hu, Y. (2022). Endophytic fungi: An effective alternative source of plant-derived bioactive compounds for pharmacological studies. Journal of Fungi, 8(2), 205. https://doi.org/10.3390/jof8020205

Yadigarova, L. (2025). Bibliometric analysis of the impact of international research collaborations: Insights from social sciences. International Scientific Journal of Universities and Leadership, (19), 37–48. https://doi.org/10.31874/2520-6702-2025-19-37-48

Yan, L., Zhu, J., Zhao, X., Shi, J., Jiang, C., & Shao, D. (2019). Beneficial effects of endophytic fungi colonization on plants. Applied Microbiology and Biotechnology, 103(8), 3327–3340. https://doi.org/10.1007/s00253-019-09713-2