Different L-Tryptophan concentrations in the formulation of bacterial bioinoculants for BRS Capiaçu seedlings
DOI:
https://doi.org/10.48017/dj.v11iSpecial_2.3950Keywords:
Plant growth-promoting bacteria, Bacterial interaction, Forage plant, Agricultural sustainabilityAbstract
*Pennisetum purpureum* (Schumach) cultivar BRS Capiaçu is notable for its high productivity, making it essential to seek technologies that improve its field management. One such technology is the application of plant growth-promoting bacteria (PGPB). This study aimed to evaluate the morphological characteristics of BRS Capiaçu seedlings following the inoculation of stem cuttings with PGPB strains isolated from *Brachiaria decumbens* Stapf. and *Brachiaria humidicola* (Rendle) Schweickerdt. The experiment followed a completely randomized design with 28 treatments: nine bacterial strains cultured in TSB medium supplemented with 0, 10, and 15 mM L-tryptophan, in addition to a control treatment involving neither inoculation nor L-tryptophan supplementation. Seedlings were kept in a germination chamber at 25 ± 5°C with a 12-hour photoperiod. Treatments containing 10 mM L-tryptophan promoted greater development of seedling morphological characteristics. Among the strains evaluated, *Sphingomonas paucimobilis* (UAGB 80), *Rhizobium cauense* (UAGB 150), *Burkholderia territorii* (UAGB 105), *Klebsiella variicola* F2R9 (UAGB 154), *Enterobacter kobei* (UAGB 119), and *Ralstonia pickettii* (UAGB 128) stood out as the most promising candidates for potential use as PGPB in BRS Capiaçu cultivation.
Metrics
References
Alves, M. J. G., Mendonça, J. J., Vitalino, G. M., Oliveira, J. P., Carvalho, E. X., Fracetto, F. J. C., Fracetto, G. G. M., & Júnior, M. A. L. (2023). Screening Digitaria eriantha cv. suvernola endophytic bacteria for maize growth promotion. Plantas, 12(14), 2589. https://doi.org/10.3390/plants12142589
Barreto, G. P., Lira, M. de A., Santos, M. V. F. dos, & Júnior, J. C. B. D. (2001). Avaliação de clones de capim-elefante (Pennisetun purpureum Schum.) e de um híbrido com o milheto (Pennisetum glaucum (L.) R. Br.) submetidos a estresse hídrico. 1. Parâmetros Morfológicos. Revista Brasileira de Zootecnia, 30, 1–6. https://doi.org/10.1590/S1516-35982001000100001
Deus, T. R. V. de., Giongo, V., Salviano, A. M., Santana, M. da S., & Silva, V. C. da., Santos, T. C. dos. (2022). Selection of green manures to provide ecosystem services in a semi-arid environment. Revista Brasileira de Ciência do Solo, 46(1), e0210105. https://doi.org/10.5327/Z2176-94781268
Elnahal, A. S. M., El-Saadony, M. T., Saad, A. M., Desoky, E. S. M., El-Tahan, A. M., Rady, M. M., & El-Tarabily, K. A. (2022). The use of microbial inoculants for biological control, plant growth promotion, and sustainable agriculture: A review. European Journal of Plant Pathology, 162(4), 1007–1007. https://doi.org/10.1007/s10658-022-02472-3
Espíndola, N. L. de., Tavares, B. M. de S., Santos, J. M. G., Barbosa, V. M. S., Santos, I. B. dos., & Oliveira, J. T. C. (2024). Inoculation of plant growth-promoting bacteria on Pennisetum purpureum Schumach cultivar BRS Capiaçu. Revista Brasileira de Ciências Ambientais, 59, e1916–e1916. https://doi.org/10.5327/Z2176-94781916
Ferreira, D. F. (2007). SISVAR: um programa para análises e ensino de estatística. Revista Symposium, 6(2), 36–41. https://do1.org/10.5327/22176-94781916
Gupta, G., Paul, S., Singh, S., Pietramellara, G., Pathan, S. I., Danish, S., Jabborova, D., Datta, R., & Jha, P. N. (2022). Exploring functional diversity and community structure of diazotrophic endophytic bacteria associated with Pennisetum glaucum growing under field conditions in a semi-arid region. Land, 11(7), 991. https://doi.org/10.3390/land11070991
Lima, D. R. M. de., Santos, I. B. dos., Oliveira, J. T. C., Costa, D. P. da., Queiroz, J. V. J. de., Romagnoli, E. M., Andreote, F. D., Freire, F. J., & Kuklinsky-Sobral, J. (2021). Genetic diversity of N-fixing and plant growth-promoting bacterial community in different sugarcane genotypes, association habitat and phenological phase of the crop. Archives of Microbiology, 203(3), 1089–1105. https://doi.org/10.1007/s00203-020-02103-7
Mesquita, A. de F. N., Bandeira, L. L., Cavalcante, F. G., Ribeiro, G. A. L., Martins, S. C. S., & Martins, C. M. (2023). In vitro co-inoculation of rhizobacteria from the semi-arid aiming at their implementation as bio-inoculants. Revista Brasileira de Ciências Ambientais, 58(1), 59–66. https://doi.org/10.5327/Z2176-94781481
Oliveira, H. P. de., Melo, R. O. de., Baldotto, M. A., Andrade, M. A., & Baldotto, L. E. B. (2018). Performance of pre-sprouted sugarcane seedlings in response to the application of humic acid and plant growth-promoting bacteria. Semina: Ciências Agrárias, 39(3), 1365–1370. https://doi.org/10.5433/1679-0359.2018v39n3p1365
Oliveira, J. T. C., Pereira, A. P. A., Souza, A. J. de., Kuklinsky-Sobral, J., Freire, F. J., Santos, M. V. F. dos., & Lira, M. D. A. (2022). Inoculation with plant-growth promoting bacteria improves seed germination and initial development of Brachiaria decumbens. Anais da Academia Brasileira de Ciências, 94(1), e20200124. https://doi.org/10.1590/0001-37652202220200124
Pandey, K., & Saharan, B. S. (2025). Soil microbiomes: a promising strategy for boosting crop yield and advancing sustainable agriculture. Discovery Agriculture, 3(1), 54. https://doi.org/10.1007/s44279-025-00208-5
Rosa, F. C., Mota, A. G., Almeida, B. L. de., Souza, G. D. S. de., Araújo, M. C., Melo, M. H. F., & Mendonça, R. D. C. M. (2023). Importância dos metabólitos secundários produzidos por actinobactérias. Revista Ciências da Saúde CEUMA, 1(1), 72–87. https://doi.org/10.61695/rcs.v1i1.6
Sharma, M., Saleh, D., Charron, J. B., & Jabaji, S. (2020). A crosstalk between Brachypodium root exudates, organic acids, and Bacillus velezensis B26, a growth promoting bacterium. Frontiers in Microbiology, 11, 575578. https://doi.org/10.3389/fmicb.2020.575578
Silva, L. S. da., Espíndola, N. L. de., Tavares, B. M. de S., Santos, J. M. G., Barbosa, V. M. S., Andrade, P. A. M. de., & Oliveira, J. T. C. (2024). Agricultural sustainability: bioinputs from bacteria as alternatives to improve the physiological and morphological development of Urochloa decumbens cv. Basilisk. Revista Brasileira de Ciências Ambientais, 59, e1980–e1980. https://doi.org/10.5327/Z2176-94781980
Taiz, L., Zeiger, E., Moller, I. M., & Murphy, A. (2017). Fisiologia e desenvolvimento vegetal (6a ed.). Artmed Editora.
Zuluaga, M. Y. A., Fattorini, R., Cesco, S., & Pii, Y. (2024). Plant–microbe interactions in the rhizosphere for smarter and more sustainable crop fertilization: the case of PGPR-based biofertilizers. Frontiers in Microbiology, 15, 1440978. https://doi.org/10.3389/fmicb.2024.1440978
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 José Matheus Gonzaga Santos, Brena Maíza de Siqueira Tavares, Vitor Mineu Silva Barbosa, Witoria Maria Cavalcante Lins, Artur Mineu da Silva Barbosa, Gabriel Oliveira Pinto, Natália Lima de Espíndola, João Tiago Correia Oliveira
This work is licensed under a Creative Commons Attribution 4.0 International License.
The Diversitas Journal expresses that the articles are the sole responsibility of the Authors, who are familiar with Brazilian and international legislation.
Articles are peer-reviewed and care should be taken to warn of the possible incidence of plagiarism. However, plagiarism is an indisputable action by the authors.
The violation of copyright is a crime, provided for in article 184 of the Brazilian Penal Code: “Art. 184 Violating copyright and related rights: Penalty - detention, from 3 (three) months to 1 (one) year, or fine. § 1 If the violation consists of total or partial reproduction, for the purpose of direct or indirect profit, by any means or process, of intellectual work, interpretation, performance or phonogram, without the express authorization of the author, the performer, the producer , as the case may be, or whoever represents them: Penalty - imprisonment, from 2 (two) to 4 (four) years, and a fine. ”
